甲苯胺藍(Toluidine Blue O)是一種常用的人工合成染料, 屬于醌亞胺染料類。這類染料主要含有胺基和醌型苯環(huán)兩個發(fā)色團，從而成色原顯色。甲苯胺藍中的陽離子有染色作用, 組織細胞的酸性物質與其中的陽離子相結合而被染色。甲苯胺藍不僅含有兩個發(fā)色團，還含有兩個助色團，能促使染料產生電離成鹽類, 幫助發(fā)色團對組織產生染色力, 使切片上的組織細胞著色，可染細胞核使之呈藍色；肥大細胞胞質內含有肝素和組織胺等異色性物質遇到甲苯胺藍可呈異染性紫紅色。

      軟骨染色液(甲苯胺藍法)呈強堿性，更利于組織細胞的著色。

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軟骨染色液(甲苯胺藍法)

軟骨染色液(甲苯胺藍法)